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NO or no may stand for:
- In common speech, the word "no" denotes negation, disagreement or unacceptance.
- New Orleans (usually N.O.)
- No symbol
- Nitric oxide (NO)
- Nobelium (No), symbol for the chemical element
- normally open switch (NO or N.O.)
- Norway, ISO country code
- Norwegian language (ISO 639 alpha-2, no)
- No!, a 2002 album by They Might Be Giants, as well as a song on that album
- Noh, a Japanese theatrical form
- №, the numero sign
- N.O. is a fictional power in the anime FLCL.