Talk:Glycolysis
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At the end of glycolysis it can proceed to either the aerobic or aneorbic in eukaryotic cells (so lactic acid builds up in your muscles when a person is sprinting). -- Eean
Data dump:
Key
1) Chimical diagram of reactants
2) Names of reactants
(reaction type & enzyme) δ G°' = ?
*critical control point
Glycolysis 1 ATP -> ADP + H+ 1) . = radioactive label
H<sub>2</sub>COH H<sub>2</sub>OCPO<sub>3</sub><sup>-2</sup>
|___O |___O
./ \ --> ./ \
|\|___ /| <--– |\|___ /|
| |
+ ATP + ADP + H+
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2) å-D-G ––---> å-D-G-6-P
(phosphorylation, *Hexokinase)
-4.0 kcal/mol ATP -> ADP + H+
Glycolysis 2
1) (P) is H2COPOsub>3</sub>-2
H2COPO3-2
|___O (P) O CH2OH
./ \ --> |/ \|
|\|___ /| <--––– \.__|/|
| |
2) å-D-G-6-P ––--> Fructose-6-P
(isomeration, phosphoglucoisomerase)
+0.4 kcal/mol
Glycolysis 3
ATP -> ADP + H+
1) (P) is H2COPO3+
(P) O CH2OH (P) O (P)
|/ \| --> |/ \|
\.__ |/| <--––– \.__|/|
| |
+ ATP + ADP + H+
2) F-6-P --> F-1,6-BP
(phoshorylation, phosphofrucoknse)
-3.4 kcal/mol ATP -> ADP + H+
Glycolysis 4
1) (P)
| (P) HC.=O
C=O | |
__ | ––– --> C=O + HCOH
|.__ <--––– | |
| __ H2C.OH (P)
|
(P)
2) F-1,6-BP --> DHAP + Gy-3-P
(Isomerization, *aldolase)
+5.7 kcal/mol
DHAP Dihydroxyacetone Phosphate
Glycolysis 5
1) Lable is mixed in 2 species
(P) HC.=O
| |
C=O --> HCOH
| <-- |
H2C.OH (P)
98% 2%
2) DHAP --> Gy-3-P
(isomerization, triose-phosphate isomerase)
+1.83 kcal/mol
Glycolysis 6
2(NAD -> NADH)
1) Double all molecules
HC.=O O=C.-OPO3-2
| --> |
HCOH <--–– HCOH
| |
(P) (P)
+ NAD + Pi + NADH + H+
2) 2(Gy-3-P --> 1,3-BP-Gy)
(dehydration, Gy-3-P dehydrogenase)
+1.5 kcal/mol NAD + Pi -> NADH
Glycolysis 7
2(ADP -> ATP)
1) Double all molecules
O=C.-OPO3-2 C.OO-
| |
HCOH --> HCOH
| <-- |
(P) (P)
+ ADP + ATP
2) 2(1,3-BP-Gy --> 3-PGy)
(Phosphoryl Transfer, phosphoglycerate kinase)
-4.5 kcal/mol 2(ADP -> ATP)
Glycolysis 8
1) Double all molecules
C.OO- C.OO-
| --> |
HCOH <-- (P)
| |
(P) H2COH
Note
2) 2(3-PGy --> 2-PGy)
(Phosphoryl Shift, phosphoglyceromutase)
+1.06 kcal/mol
Note: 2-PGy's (P) has 1 fewer H than referance (P).
Glycolysis 9
---> + 2H2O
1) Double all molecules (Note 1)
C.OO- C.OO-
| – --> |
(P) <-- (P)
| ||
H2COH CH2
Note + H2O
2) 2(2-P-Gy ----> phosphoenol Py)
(Dehydration, enolase)
1st molecule's (P) has 1 fewer H than referance (P).
2nd molecule's (P) has 2 fewer H than normal (P)
Glycolysis 10
2(ADP -> ATP)
1) Double all molecules (Note 1)
C.OO- C.OO-
| – --> |
(P) <--– C=O
|| |
CH2 CH3
+ ADP + ATP
2) 2(PEP --> pyruvate)
(Phosphoryl Transfer, ) ADP -> ATP
-7.5 kcal/mol
Note: 1st molecule's (P) has 2 fewer H than normal (P)
TODO: Double check to make sure this is correct, try to translate into English, put into right justified table with text of article flowing on the left. --maveric149
Well spent
Not certain if the references to energy being 'well spent', etc during the energy investment phase are entirely appropriate. Seems to me to be a little unscientific. Stryer states that glycolysis is the sequence of reactions that converts glucose into pyruvate with the concommitant production of ATP. Based on this I don't think that Entner-Doudoroff can be considered a form of glycolysis. As far as I'm aware, glycolysis is Embden-Meyerhof. Could anyone confirm the differences between hexokinase and glucokinase? My information is hazy. I think maybe pathway section needs to be split - one for the pure reactions and then maybe a seperate one for the implications of the reactions. ie; that phoshorylation of glucose leads to instabliity that allows it to be split. I am unable to satisfactorily integrate the process and the reasons individual steps are carried out. Think this is on the way to being a really good article. All comments very very welcome. I'd like to hear your thoughts.Marc Isaacs
Welcome, and nice work at first glance. I have written an extensive article on glucokinase and fleshed out the article on hexokinase, so I would value your opinion as to whether they adequately answer your question about the differences. Many of our articles on carbohydrate metabolism could be improved, or at least made more physiological so there is some context for the reactions. alteripse 11:56, 1 Jun 2005 (UTC)
Hi. I'll look through those articles when I have a bit more time to study them properly. You're right - the glucokinase one certainly is extensive :o). I think what you say about making this article more physiological is a very good point. With this in mind, perhaps there is potential for a section on regulation of the process? This could allow for a lot of detail to be removed from the pathway section, making that a bit more streamlined. What do you think? Marc Isaacs 14:33, 1 Jun 2005 (UTC)
It seems that glycolysis is indeed sometimes used to include ED. See, for exampe, these articles (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=11271421,9075622,2254282,3611027). I'm not sure why you think ED is inconsistent with Stryer's definition (is it because he says "the sequence of reactions" rather than "any sequence of reactions"? since there isn't just one, his definition is ambiguous, at best), but I wouldn't base this call on a definition in an introductory text. With that said, I note that people sometimes clearly discuss glycolysis on the one hand and ED on the other. Josh Cherry 15:25, 4 Jun 2005 (UTC) Josh, could you please provide PMID rather than link like this, as I don't get anything when I click on your link except the NLM page with no query. alteripse 15:31, 4 Jun 2005 (UTC)
- Oops, I messed up the link. It's fixed now. Josh Cherry 16:08, 4 Jun 2005 (UTC)