Locked nucleic acid
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A locked nucleic acid (LNA) is a modified RNA nucleotide. LNA increases the thermal stability (melting temperature) of oligonucleotides. This can be used to increase the sensitivity and specificity of expression DNA microarrays, FISH probes and other techniques based on oligonucleotides. For the detection of miRNA the use of LNA is currently (2005) the only efficient method.
External links
- A predicter for the physical properties of LNA modified oligonucleotides (http://lnatools.com)
- LNA (locked nucleic acid): high-affinity targeting of complementary RNA and DNA (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15491130)
- LNA: a versatile tool for therapeutics and genomics (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12573856)
- Alternative Nucleic Acid Analogues for Programmable Assembly: Hybridization of LNA to PNA (http://pubs.acs.org/cgi-bin/sample.cgi/nalefd/2005/5/i01/abs/nl048246f.html)