Selective medium
From Academic Kids
A selective medium is a substance (usually agar-based) which grows a specific type of microbe. Using different nutrient ratios, chemical compounds (e.g. methylene blue) and incubation times, speciation of bacteria can be performed before to use other methods for the presuntive identification of bacteria or fungus.
Some examples of selective media are:
- Eosin-methylen blue agar (EMB) that contains methylene blue which is toxic for Gram-positive bacteria allowing only the growth (thus selecting) of Gram negative bacteria.
- YM (Yeast and Mold) which has a low pH, deterring bacterial growth.
- Blood agar (used in strep tests), which contains beef heart blood which becomes transparent in the presence of hemolytic Streptococcus.
- MacConkey's Agar for Gram-negative bacteria.
[edit]
